Purpose This study examined the activation of the rectus abdominis(RA), external oblique abdominal(EOA). multifidus muscles while stabilization exercise was performed in a four point kneeling position. Methods Experiments were conducted on 10 healthy male adult, 10 healthy female adult. who voluntarily agreed to participate in the study. Each subject was instructed regarding maximal voluntary isometric contractions(MVIC) and stabilization exercise in four point kneeling position. Results In position 1(four point kneeling position), external oblique abdominis and multifidus showed more significant increase than rectus abdominis statisticall. In position 2(lifting right arm and left leg), external oblique abdominis showed more significant increase than multifidus and rectus abdominis statistically. In position 3(four point kneeling using gym ball), multifidus showed more significant increase than external oblique abdominis and rectus abdominis statistically. Figure of MVIC made no great difference between male and female statistically. Conclusion Selecting an optimal position can aid subjects on physical conditions while performing stabilization exercises in the four point kneeling position.