Purpose Deep squatting is a common position for Asians during activities of daily living. The purpose of this study is to investigate that ankle joint could contribute during deep squatting. Methods 44 healthy normal participants(20 men, 24 women) were recruited for this study. Whether they were able to perform the deep squat position with their heels on the ground or not, participants allocated into two groups (referred to as heels on ground(HG) squat(n=22(13 men, 9 women)), heels off(HO) squat(n=22(7 men, 15 women)). Results In this study, active/passive ankle joint dorsiflexion range of motion and static balance were assessed. There were significant difference in ankle joint dorsiflexion ROM except right ankle passive ROM. Also heels off squat group showed a significant reduction in static balance. Conclusion These findings show that individuals who can’t perform the deep squat position exhibit reduced ankle ROM and static balance. Thus, deep squat position might be recommended as a simple task to evaluate ankle joint flexibility and static balance.