Purpose The aim of this study was to identity the effects of eccentric activation training of plantar flexors on balance and gait in patients with stroke. Methods A total of 30 patients were randomly allocated into two groups: The study group eccentric activation training of plantar flexors (n=14), and the control group conventional training (n=16). Both groups received equal amount of therapy sessions for 30 minutes a day, 3 times a week for 8 weeks. Results The study group showed significant changes of ankle range of motion in active dorsi flexion (p<0.05). The study group showed significant changes in the standing balance. There were significant increased in foot pressure(p<0.05). The study group showed significant changes in velocity, cadence, NP step length, P step length, NP stance time, P stance time compared to the control group(p<0.05). Conclusion Based on results of this study, the eccentric activation of plantar flexors was effective therapeutic approach in improving ankle range of motion, balance, gait for stroke patients.