Purpose The purpose of this study was to investigate the effects of Bilateral activities intensive training on muscle activity, upper extremity function in stroke patients. Methods Thirty stroke patients were admitted to B hospital in Suwon, Gyeonggido, and the intervention program was divided into two groups, 15 for Bilateral activities intensive training and Unilateral activities intensive training, respectively. The intervention program was conducted four times a week for four weeks, and the affected side upper extremity muscle activity and upper extremity function were evaluated two times before and after the experiment. Results Bilateral activities intensive training group, there was a statistically significant difference in upper extremity muscle activity and upper extremity function before and after intervention. In the Unilateral activities intensive training group, there was a statistically significant difference before and after intervention in the upper extremity muscle activity except ant.deltoid and upper extremity function evaluation except MALHS. There was statistically significant difference between the two groups, in triceps and extensor carpi radialis in the upper extremity muscle activity, and a statistically significant difference in FMAUE, BBT, MALAS, HW in the evaluation of upper extremity function. Conclusion Bilateral activities intensive training is more effective than Unilateral activities intensive training for stroke patients to use both hands functionally. Bilateral activities intensive training was more effective than Unilateral activities intensive training to improve muscle activity and improve upper limb function and hand agility in the distal upper extremities of stroke patients.