Purpose This study was to investigate the effects of ankle control training using neuromuscular electrical stimulation (NMES) on ankle joint mobility and balance in a healthy adult. Methods This study was designed the ABA format in a single subject research design. The subject was a healthy woman aged 35. The intervention was carried out the NMES on gastrocnemius muscle in upright standing. The intervention was performed for 30 minutes a day, 5 times a week, for four weeks. The effectiveness of the intervention was measured before each session with the ankle range of motion (ROM), knee to wall test (KWT), functional reach test (FRT), sway index (SI), foot pressure (FP) and foot center of pressure (FCOP) distance. A two standarddeviationband method was used to evaluate improvement. Results As the result of the ROM, FRT and KWT, there have been improvements on ankle planter flexor muscle length and balance during intervention and regression period. SI show declines in standing position on firm pad with closed eyes. FP show improvements in left heel pressure in bipedal position. FCOP distance in sit to standing show improvements. FCOP distance in single leg standing position and functional reach test show declines. Conclusion Ankle control training using NMES was effective in ankle mobility and balance.