Purpose The purpose of this study is to compare the difference in lower extremity muscle activity according to walking condition with real environment and virtual reality and found the effect of virtual reality condition over time.  Methods The participants were 15 male university students in Busan. The experiment was conducted in a real environment with two conditions: 0 degree walking and 10 degrees, and three conditions: VR 10 degree walking (Walking simulator, Steam platform) by wearing VR (Vive pro 2 headset, Vive Company), and walking over time for 10 and 30 minutes in VR. All walking was done at 4 km/h for 1 minute and 30 seconds. An electromyography device (4DMT, BrowSrping, Korea) was used to measure rectus femoris, biceps femoris, tibialis anterior, and gastrocnemius according to each walking condition. Results In a real environment, there was no difference in the activity of each muscle according to 0 degree walking and 10 degree walking condition, but the muscle activity was high in the biceps femoris, gastrocnemius. There was no difference between the 10degree walking condition in the real environment and the 10degree walking condition in virtual reality. There was no statistically significant change in muscle activity during walking immediately after wearing VR, 10 minutes later, and 30 minutes later.  Conclusion Since there was no between real environment and a VR environment, it is thought that walking training in a convenient VR environment can increase the activity of the leg muscles and maintain or strengthen muscle strength.