Purpose The purpose of this study was to investigate the effects of mat Pilates exercise on TUG variables and gait in elderly women. Methods The subjects of this study were 24 people, 12 in the mat Pilates experimental group and 12 in the control group who did not receive any intervention. The experimental group received the intervention for 60 minutes twice a week for 8 weeks. TUG variables and gait were assessed using the Kinesis QTUG device before and after the intervention. Result The results of this study showed that in the experimental group, there were significant differences in TUG variables such as TUG evaluation completion time, Sit tostand time, time to turn, Standtosit time, walk time, stride velocity, and Stride length (p<0.05), and a significant difference was also found between the experimental group and the control group (p<0.05). Conclusion The results of this study showed that mat Pilates exercise applied to the elderly for 8 weeks had a positive effect on TUG variables and gait, and it is considered an appropriate intervention method for reducing fall risk factors and increasing gait ability even with short term exercise.